Novel assays for small molecule-precursor miRNA binding
- Reference number
- ID14-0036
- Start and end dates
- 150101-191231
- Amount granted
- 2 500 000 SEK
- Administrative organization
- Göteborg University
- Research area
- Life Sciences
Summary
The overall goal is to develop in vitro assays suitable for monitoring small molecule binders to precursor miRNA, using internally placed fluorescent RNA base analogues (fRNAba) as FRET-pairs. The project includes to develop efficient protocols for preparation of fRNAba with properties useful for very high sensitivity and even single molecule FRET experiments; to develop efficient protocols for preparation of protected fRNAba-ribo-phosphoramidite building blocks and their site-specific incorporation of these building blocks into RNA by solid-phase RNA synthesis; and to use these molecules in development and validation of small molecule-precursor miRNA binding assays based on using internally placed fRNAba as FRET-pairs. The project is a collaborative and interdisciplinary project between AstraZeneca, Prof. Morten Grötli (UGOT) and Prof. Marcus Wilhelmsson (Chalmers), that will train one PhD student state of the art RNA chemistry.
Popular science description
The overall goal is to develop in vitro assays suitable for monitoring small molecule binders to precursor miRNA, using internally placed fluorescent RNA base analogues (fRNAba) as FRET-pairs. The project includes to develop efficient protocols for preparation of fRNAba with properties useful for very high sensitivity and even single molecule FRET experiments; to develop efficient protocols for preparation of protected fRNAba-ribo-phosphoramidite building blocks and their site-specific incorporation of these building blocks into RNA by solid-phase RNA synthesis; and to use these molecules in development and validation of small molecule-precursor miRNA binding assays based on using internally placed fRNAba as FRET-pairs. The project is a collaborative and interdisciplinary project between AstraZeneca, Prof. Morten Grötli (UGOT) and Prof. Marcus Wilhelmsson (Chalmers), that will train one PhD student state of the art RNA chemistry.